| 质粒名称: | pRetroX GFP Mps1 wt |
|---|---|
| 出品公司: | addgene |
| 目录编号: | 63702 |
| 存储实验室: | Floris Foijer |
|
载体骨架基本信息 |
|
| 载体名称: | pRetrox |
| 载体出品公司: | Clontech |
| 空载体大小: | 7305 bp |
| 完整质粒大小: | 9879bp |
| 载体类型: | 哺乳细胞表达、Retroviral |
| 筛选标记: | Puromycin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Mps1 / TTK / ESK / PYT |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 2574 bp |
| 基因突变: | T2A, S13L, A92T, V104T, V114I, E161K, Y193K, F198Y, D202T, I203V, Y258F, A283V, K288T, N290Y relative to RpBphP2 |
| GenBank编号 | 7272 |
| 启动子: | tight TRE promoter |
| 标签/融合蛋白: | EGFP (N terminal on insert) |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | BamHI(未破坏) |
| 3’克隆位点: | NotI (未破坏) |
| 5’测序引物 | EGFP-C |
| 3’测序引物 | MSCV-rev |
|
文献引用方法 |
|
| 材料和方法部分: | pRetroX GFP Mps1 wt was a gift from Floris Foijer (Addgene plasmid # 63702 ; http://n2t.net/addgene:63702 ; RRID:Addgene_63702) |
| 参考文献部分: | Chromosome instability induced by Mps1 and p53 mutation generates aggressive lymphomas exhibiting aneuploidy-induced stress. Foijer F, Xie SZ, Simon JE, Bakker PL, Conte N, Davis SH, Kregel E, Jonkers J, Bradley A, Sorger PK. Proc Natl Acad Sci U S A. 2014 Sep 16;111(37):13427-32. doi: 10.1073/pnas.1400892111. Epub 2014 Sep 2. 10.1073/pnas.1400892111 PubMed 25197064 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pRetroX GFP Mps1 wt 9879 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Inducible expression of GFP-Mps1 fusion in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pRetroX GFP Mps1 wt
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9879)
AUTHORS Foijer F, Xie SZ, Simon JE, Bakker PL, Conte N, Davis SH, Kregel E,
Jonkers J, Bradley A, Sorger PK
TITLE Chromosome instability induced by Mps1 and p53 mutation generates
aggressive lymphomas exhibiting aneuploidy-induced stress.
JOURNAL Proc Natl Acad Sci U S A. 2014 Sep 16;111(37):13427-32. doi:
10.1073/pnas.1400892111. Epub 2014 Sep 2.
PUBMED 25197064
REFERENCE 2 (bases 1 to 9879)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9879
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 309..512
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 463..483
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 513..688
/label=5' LTR (truncated)
/note="truncated long terminal repeat from Moloney murine
sarcoma virus"
misc_feature 751..950
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 1151..1567
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 1497..1519
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 1535..1551
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
promoter 1603..1917
/label=tight TRE promoter
/note="Tet-responsive promoter PTight, consisting of seven
tet operator sequences followed by the minimal CMV
promoter"
protein_bind 1611..1629
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 1647..1665
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 1682..1700
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 1718..1736
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 1754..1772
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 1789..1807
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 1825..1843
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
primer_bind 1890..1914
/label=LNCX
/note="Human CMV promoter, forward primer"
CDS 1931..2647
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 1931..2647
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(1976..1997)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(2237..2256)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 2584..2605
/label=EGFP-C
/note="EGFP, forward primer"
promoter 5285..5784
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(5320..5343)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 5717..5736
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
CDS 5805..6404
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(5805..5824)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 6301..6321
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
LTR 6585..7010
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
Moloney murine leukemia virus"
primer_bind complement(7113..7135)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(7271..7291)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 7276..7605
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 7456..7591
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 7518..7537
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind 7627..7646
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(7727..7744)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7898..8486)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7978..7997)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8657..9517)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9280..9299
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9518..9622)
/gene="bla"
/label=AmpR promoter
primer_bind 9690..9708
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
enhancer 9808..308
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
ORIGIN
1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
1561 aggcgccgga attgaagatc tgaggccctt tcgtcttcac tcgagtttac tccctatcag
1621 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgat gtcgagttta
1681 ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt
1741 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgagtttatc cctatcagtg
1801 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgaggtagg
1861 cgtgtacggt gggaggccta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
1921 agaaggatct atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
1981 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
2041 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
2101 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
2161 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
2221 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
2281 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
2341 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
2401 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
2461 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
2521 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
2581 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
2641 gtacaaggga ggacccggag gaggatccat ggaatccgag gatttaagtg gcagagaatt
2701 gacaattgat tccataatga acaaagtgag agacattaaa aataagttta aaaatgaaga
2761 ccttactgat gaactaagct tgaataaaat ttctgctgat actacagata actcgggaac
2821 tgttaaccaa attatgatga tggcaaacaa cccagaggac tggttgagtt tgttgctcaa
2881 actagagaaa aacagtgttc cgctaagtga tgctctttta aataaattga ttggtcgtta
2941 cagtcaagca attgaagcgc ttcccccaga taaatatggc caaaatgaga gttttgctag
3001 aattcaagtg agatttgctg aattaaaagc tattcaagag ccagatgatg cacgtgacta
3061 ctttcaaatg gccagagcaa actgcaagaa atttgctttt gttcatatat cttttgcaca
3121 atttgaactg tcacaaggta atgtcaaaaa aagtaaacaa cttcttcaaa aagctgtaga
3181 acgtggagca gtaccactag aaatgctgga aattgccctg cggaatttaa acctccaaaa
3241 aaagcagctg ctttcagagg aggaaaagaa gaatttatca gcatctacgg tattaactgc
3301 ccaagaatca ttttccggtt cacttgggca tttacagaat aggaacaaca gttgtgattc
3361 cagaggacag actactaaag ccaggttttt atatggagag aacatgccac cacaagatgc
3421 agaaataggt taccggaatt cattgagaca aactaacaaa actaaacagt catgcccatt
3481 tggaagagtc ccagttaacc ttctaaatag cccagattgt gatgtgaaga cagatgattc
3541 agttgtacct tgttttatga aaagacaaac ctctagatca gaatgccgag atttggttgt
3601 gcctggatct aaaccaagtg gaaatgattc ctgtgaatta agaaatttaa agtctgttca
3661 aaatagtcat ttcaaggaac ctctggtgtc agatgaaaag agttctgaac ttattattac
3721 tgattcaata accctgaaga ataaaacgga atcaagtctt ctagctaaat tagaagaaac
3781 taaagagtat caagaaccag aggttccaga gagtaaccag aaacagtggc aatctaagag
3841 aaagtcagag tgtattaacc agaatcctgc tgcatcttca aatcactggc agattccgga
3901 gttagcccga aaagttaata cagagcagaa acataccact tttgagcaac ctgtcttttc
3961 agtttcaaaa cagtcaccac caatatcaac atctaaatgg tttgacccaa aatctatttg
4021 taagacacca agcagcaata ccttggatga ttacatgagc tgttttagaa ctccagttgt
4081 aaagaatgac tttccacctg cttgtcagtt gtcaacacct tatggccaac ctgcctgttt
4141 ccagcagcaa cagcatcaaa tacttgccac tccacttcaa aatttacagg ttttagcatc
4201 ttcttcagca aatgaatgca tttcggttaa aggaagaatt tattccatat taaagcagat
4261 aggaagtgga ggttcaagca aggtatttca ggtgttaaat gaaaagaaac agatatatgc
4321 tataaaatat gtgaacttag aagaagcaga taaccaaact cttgatagtt accggaacga
4381 aatagcttat ttgaataaac tacaacaaca cagtgataag atcatccgac tttatgatta
4441 tgaaatcacg gaccagtaca tctacatggt aatggagtgt ggaaatattg atcttaatag
4501 ttggcttaaa aagaaaaaat ccattgatcc atgggaacgc aagagttact ggaaaaatat
4561 gttagaggca gttcacacaa tccatcaaca tggcattgtt cacagtgatc ttaaaccagc
4621 taactttctg atagttgatg gaatgctaaa gctaattgat tttgggattg caaaccaaat
4681 gcaaccagat acaacaagtg ttgttaaaga ttctcaggtt ggcacagtta attatatgcc
4741 accagaagca atcaaagata tgtcttcctc cagagagaat gggaaatcta agtcaaagat
4801 aagccccaaa agtgatgttt ggtccttagg atgtattttg tactatatga cttacgggaa
4861 aacaccattt cagcagataa ttaatcagat ttctaaatta catgccataa ttgatcctaa
4921 tcatgaaatt gaatttcccg atattccaga gaaagatctt caagatgtgt taaagtgttg
4981 tttaaaaagg gacccaaaac agaggatatc cattcctgag ctcctggctc atccatatgt
5041 tcaaattcaa actcatccag ttaaccaaat ggccaaggga accactgaag aaatgaaata
5101 tgttctgggc caacttgttg gtctgaattc tcctaactcc attttgaaag ctgctaaaac
5161 tttatatgaa cactatagtg gtggtgaaag tcataattct tcatcctcca agacttttga
5221 aaaaaaaagg ggaaaaaaat gagcggccgc gccggctcta gatcgcgaac gcgtgaattc
5281 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg
5341 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt
5401 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta
5461 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta
5521 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc
5581 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga
5641 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg
5701 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc
5761 tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatgacc gagtacaagc
5821 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
5881 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
5941 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
6001 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
6061 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
6121 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
6181 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
6241 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
6301 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
6361 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc
6421 cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc ggcgtctcga gatatcagtg
6481 gtccaggctc tagttttgac tcaacaatat caccagctga agcctataga gtacgagcca
6541 tagataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc
6601 tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa aaatacataa
6661 ctgagaatag agaagttcag atcaaggtca ggaacagatg gaacagggtc gaccctagag
6721 aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa
6781 ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa
6841 aagagcccac aacccctcac tcggggcgcc agtcctccga ttgactgagt cgcccgggta
6901 cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg
6961 ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcatt tgggggctcg
7021 tccgggatcg ggagacccct gcccagggac caccgaccca ccaccgggag gtaagctggc
7081 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
7141 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
7201 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtag
7261 atccggctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
7321 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
7381 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
7441 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
7501 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
7561 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tactggctta
7621 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
7681 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
7741 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
7801 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
7861 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
7921 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
7981 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
8041 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
8101 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
8161 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
8221 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
8281 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
8341 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
8401 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
8461 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
8521 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
8581 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
8641 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
8701 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
8761 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
8821 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
8881 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
8941 ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt
9001 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
9061 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
9121 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
9181 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
9241 tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca
9301 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
9361 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
9421 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
9481 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
9541 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
9601 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
9661 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc
9721 aagaattagc ttggccattg catacgttgt atccatatca taatatgtac atttatattg
9781 gctcatgtcc aacattaccg ccatgttgac attgattatt gactagttat taatagtaat
9841 caattacggg gtcattagtt catagcccat atatggagt
//
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